Green Synthesis and Characterization of Zinc Oxide Nanoparticles using Fragaria Anannsa extract and their Antioxidant and Antimicrobial Activities

BACKGROUND The global threat of urinary tract infections and antimicrobial resistance towards urinary pathogens emphasizes the need for alternative treatments against evolving pathogens.This study focuses on synthesizing zinc oxide nanoparticles (ZnO NPs) using a simple, cost-effective, and eco-friendly green approach using extracts from Fragaria x ananassa (strawberry) for antimicrobial and antioxidant properties. OBJECTIVES To synthesize and evaluate Fragaria x ananassa -mediated zinc oxide nanoparticles for their antimicrobial and antioxidant activities. METHODS Fragaria x ananassa ZnO nanoparticles were synthesized by blending 400mL of deionized water with 400mL of 400% (w/v) fruit extract at 30°C to prepare a 2M zinc acetate solution. Following filtration and drying at 80°C, the resulting powder was transferred into Eppendorf tubes for Characterization. The formation of ZnONPs was confirmed by ultraviolet- visible spectroscopy. The morphology and composition of prepared NPs were determined by Scanning electron microscopy(SEM), X-ray diffraction (XRD), Energy Dispersive X-ray analysis (EDX), and Fourier-transform infrared spectroscopy(FT-IR). The antioxidant activity was assessed using the DPPH scavenging assay. Finally, antimicrobial activities were examined against clinical isolates of urinary pathogens. RESULTS The formation of ZnO nanoparticles was confirmed through the UV- Vis spectroscopy and a surface-plasmon resonance peak was observed at 205 nm. The particles were stable, closely packed, and irregular with a mean size of 14.92 nm. FTIR analysis showed a capping agent absorbed onto the surface of the nanoparticle. ZnO NPs also enhanced antioxidant activity due to the activation of reactive oxygen species (ROS). Overall, the outcomes of antimicrobial studies revealed that smaller particles demonstrated greater effects against pathogens. CONCLUSION Zinc Oxide Nano Particles were successfully synthesized using Fragaria x ananassa extract as an antimicrobial agent, reducing and capping agent.