Evaluation of the dose-dependent toxicity in different classes of NSAIDs on rodents with special reference to tissue architecture

The main objective of this study is to make an assessment of the various types of NSAIDs toxicity on rodents, depending on the amount of the drug used, and with a special focus on tissue architecture. The mice were split into groups of six: group 1 was given normal saline, and group 2,3,4,5 and 6 were given orally through the feeding needle and 1 ml syringe with a single dose of Aspirin, Diclofenac, Paracetamol, Ibuprofen, and Disprin at concentrations of 1 and 2 mg/kg BW, respectively, for 10 days daily. On the 11th day of the experiment, the mice were killed by cervical dislocation. The study found that there was a significant change in BW due to the administration of NSAIDs at all concentrations in comparison with the saline group. The liver was observed as a whole in its cell death that was reversible, with damaged hepatocytes, necrosis, and shrunken central vein. The liver margins were lost due to the death of hepatocytes and the disorientation of the cartwheel arrangement of hepatocytes. When the drugs were given, the liver showed hyperchromatic cells with intermedial dysplastic cells, sinusoidal space marginal increase, and damaged hepatocytes. The kidney showed shrunken Bowman’s capsule, medullary rays, enlarged Bowman’s capsule, damaged Bowman’s capsule, and inflamed Bowman’s capsule. The present study concludes that there is a dose-dependent detrimental effect of an intake of NSAIDs and confirmed that, by increasing the concentration of the NSAIDs the toxicity was elevated at the tissue level.