Comparison of Quality Attributes of Gastrodia elata Grown by Artificial Mushroom Stick Substitution and Traditional Wood Cultivation

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Abstract

Facing wild resource depletion, artificial cultivation of Gastrodia elata ( G. elata ) is crucial. This study compared traditional wood (TW) and artificial substrate (AM) cultivation impacts on G. elata quality. High-Performance Liquid Chromatography (HPLC) showed similar gastrodin levels, but TW G. elata had higher 4-hydroxybenzylalcohol (0.05%-0.16%) than AM G. elata (0.02%-0.04%), while AM G. elata had more parishin E (0.90%-1.58%) than TW G. elata (0.68%-0.96%). AM G. elata exhibited elevated crude protein (8.86%-10.57%) and stable total polysaccharides (38.91%-39.90%), contrasting with TW G. elata . Umami amino acids (glutamic acid, aspartic acid) were significantly higher in AM G. elata (6.03–7.37 mg/g, 1.24–1.88 mg/g). Headspace Solid-Phase Microextraction coupled with Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS) identified thirteen differential volatile compounds, with AM G. elata showing increased esters and aromatics like (2S,3S)-2,3-butanediol and tetramethylpyrazine, contributing sweet/meaty notes. TW G. elata was rich in decanal and (2E,4E)-deca-2,4-dienal, yielding citrus/chicken-like aromas. AM cultivation enhances G. elata ’s nutritional and aromatic profiles, supporting its refined application.

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