Cultivation of Taraxum officinale using a temporary immersion bioreactor system (TIBS)

Taraxacum officinale F. H. Wigg. ( T. officinale ), a perennial herb of the genus Taraxacum in the family Asteraceae, is widely recognized for its dual edible and medicinal properties. It has been traditionally used to treat various inflammatory conditions and exhibits significant anti-inflammatory activities. In this study, the tissue culture and rapid propagation of T. officinale were carried out using the temporary immersion bioreactor system (TIBS). By optimizing the immersion frequency, inoculum density and sucrose concentration, an efficient reactor-based culture system was established. The optimal conditions were determined to be an immersion frequency of 5 min/6 h, an inoculum density of 60 plantlets·L ^− 1 , and the sucrose concentration of 20 g·L ^− 1 . Compared with the traditional solid-state culture technique, reactor-based culture demonstrated significant advantages in plant growth morphology, proliferation rate and taraxasterol content. This research serves as a foundational framework for the mass production of taraxasterol.