Pleurotus ostreatus modulates the intestinal glucose absorption by the Na(+)/glucose cotransporter 1 (SGLT1) in C57BL/6 mice

The use of medicinal mushrooms for the treatment of glycemic metabolism disorders has been extensively tested as a therapeutic alternative. The aim of this study was to analyze the effect of Pleurotus ostreatus extract on intestinal glucose absorption mediated by Na(+)-glucose cotransporter 1 (SGLT1) in C57BL/6 mice. C57BL/6 mice were administered P. ostreatus extract (2 g/kg) by gavage for three weeks. Blood glucose levels and gene expression of various proteins involved in intestinal glucose uptake were then analyzed. We found that blood glucose levels increased significantly after administration of P. ostreatus , an effect that was abolished by the SGLT1 inhibitor. Although the three-week exposure to the extract did not alter the expression of the Sglt1, Glut2 and Glu5 genes in the proximal intestine of the mice, it increased the expression of the atp1a1 gene. To investigate the possible effects of the extract on SGLT1, a molecular docking analysis was performed. The binding affinity between the six main components of the extract and the SGLT1 receptor was examined. It was found that naringin and rutin had the strongest binding affinity. Our results suggest that repeated ingesture of P. ostreatus extract sensitizes SGLT1 and promotes increased gene expression of atp1a1 without affecting blood glucose metabolism.