Craterilacuimicrobium aquaticum gen. nov. sp. nov., a novel member of the order Neisseriales isolated from crater lake

Strain TC5R-5 ^T , a white rod-shaped bacterium, was isolated from Aershan Tianchi crater lake in northern China. It is Gram-stain-negative, catalase- and oxidase- positive. Optimal growth occurs at 20–30°C, pH of 6.0–7.0 and in the presence of 0-0.5% (w/v) NaCl. Based on 16S rRNA gene sequences analysis, strain TC5R-5 ^T exhibits the highest similarity (94.3%) to Chromobacterium alkanivorans IITR71 ^T . The genomic DNA G + C content of strain TC5R-5 ^T is 51.4% according to the genome sequence. The predominant cellular fatty acids are summed feature 3 (comprising C _16:1 ω 7 c /C _16:1 ω 6 c ) and C _16:0 . The predominant quinone is ubiquinone Q-8, with Q-7 present in moderate amounts. The polar lipid profile comprises phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), an unidentified aminophospholipid (APL), and an unidentified phospholipid (PL). Through polyphasic taxonomy and molecular phylogenetic analysis, strain TC5R-5 ^T is proposed to represent a novel species of a new genus of the family Chromobacteriaceae within the order Neisseriales , for which the name Craterilacuimicrobium aquaticum gen. nov. sp. nov. is proposed. The type strain is TC5R-5 ^T (= CGMCC 1.17058 ^T  = KCTC 72736 ^T ).