A 2D/3D Nanoplatform for Electrochemical Diagnosis of miRNA-122 in Breast Cancer Cell Lines
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A novel, label-free electrochemical biosensor was precise engineered for the ultrasensitive and selective detection of miRNA-122, a pivotal biomarker in breast cancer diagnostics. This innovative sensing platform harnesses the exceptional conductivity of Ti 3 C 2 MXene nanosheets, synergistically integrated with Au-decorated NH 2 -UiO-66 metal-organic frameworks, yielding a structurally stable and electrochemically active hybrid interface. A thiolated DNA capture probe was immobilized onto the gold-modified MOF, enabling precise hybridization with the target miRNA-122. Methylene blue functioned as a robust redox indicator, exhibiting distinct electrochemical shifts upon duplex formation through π–π stacking and electrostatic interactions. The biosensor demonstrated outstanding analytical performance with dual linear response ranges (1 pM–1 nM and 1 nM–750.0 nM) and an impressively low limit of detection of 503 fM. High sequence specificity was achieved, successfully differentiating miRNA-122 from non-complementary and closely related sequences. Validation with lysates from three distinct breast cancer cell lines (MCF-7, MDA-MB-231, and SKBR3) confirmed its efficacy in complex biological matrices. Altogether, this biosensor platform offers a powerful and reliable tool for early breast cancer detection and holds substantial potential for clinical applications in miRNA-based diagnostics.
