Software Assisted Personalized Transduction Platform for Serum Biomarker Detection based on Electrochemically Engineered Cr- MOF - Graphene Oxide Nanohybrid

Incorporation of versatile hybrid frameworks with high surface area 2D materials can be an interesting approach for the development of sensitive biosensing platforms. A single step electrodeposition of chromium metal organic framework (Cr-MOF) and its nanoengineering through graphene oxide (GO) incorporation has been attempted in the study for the immune-sensing of alkaline phosphatase (ALP). ALP being a crucial biomarker with its close association to health abnormalities including liver disease, bone disorder, and kidney damage can be an important area for diagnosis. Here, Cr-MOF has been incorporated with GO for the bioconjugation of Anti-ALP to facilitate selective binding and detection of ALP. The developed probe has been thoroughly characterized through various physical and electrochemical techniques for the confirmation of synthesis and binding events of the final probe with the target analyte. This unveiled good analytical capability with a wide linear dynamic range from 50 U/L to 1000 U/L and detection limit of 2.64 U/L, covering the clinically relevant range. This platform has also been tested against categories of potential interferants ranging from ions to biomolecules along with a real sample testing in serum sample with a proven recovery between 86 to 98%. Further, the developed probe was integrated with software for data analysis and providing user-friendly approach for ALP detection. This study can further be improvised to deploy in the primary healthcare centres / remote areas for more affordable diagnosis.