Antibacterial Activity Of Silver Nanoparticle- A prospective study

This paper presents the antibacterial activity of Silver nanoparticles. Silver nanoparticles (AgNp) can invade the walls of bacteria cells, altering the composition of cell membranes and potentially causing cell death. Their effectiveness results from their small size (nanoscale) and high surface area compared to the volume ratio. By releasing silver ions, they can cause reactive oxygen species to be produced, which can enhance the permeability of cellular membranes and therefore stop the replication of deoxyribonucleic acid. Silver nanoparticle antibacterial mechanism has been implemented for various therapeutic applications including medical prosthetic devices. In this study, the Salmonella Green Fluorescence Protein (GFP) & Salmonella Typhimurium bacterial strains were encapsulated with different doses of nanoparticle & perceived Microbial Growth, Zone of Inhibition by well diffusion test. Salmonella GFP under Fluorescence & Bright field Microscope concerning Trisodium Citrate (TSC) concentration have been observed. Here, the antibacterial impact of Silver nanoparticles before & after the dialysis process was also contrasted. The result from the Microbial Growth curve & well diffusion test demonstrates that with increasing the dose of AgNp bacterial growth was minimal or antibacterial efficacy became more. However, less Zone of Inhibition after the dialysis process of AgNp solution was observed than before dialysis by which excess salt & reducing agent removes were analyzed. Furthermore, the Salmonella bacterial aggregation in low TSC in contrast, no aggregation has been perceived in terms of high concentration of TSC by investigating with fluorescence & bright field microscope found. All these investigations indicate the antibacterial impact of AgNp.