Liposome integrated hydrogel for diabetic wound healing: Molecular mechanisms in inflammation and oxidative stress mitigation

The present study aimed to formulate p-CA liposomes loaded hydrogel for effective wound healing. The preparation and optimization of liposomes is done by ethanol injection method using 3 ² factorial design. The effect of variables that is lipid and cholesterol concentration on particle size and entrapment efficiency (%) was investigated. Prepared liposomes were characterized for zeta potential, morphology, in-vitro release and ex-vivo permeation study. The optimized liposomes exhibited particle size of 184.5nm with zeta potential value of -24.9mV and highest entrapment efficiency of 91%. The liposomes were loaded in gel using 934P. Liposomal loaded gel displayed higher permeation of 73.41% compared to plain gel (49.62%). Wound healing study demonstrated remarkable healing with more than 98% wound closure by liposomal hydrogel which confirms good wound contraction in both diabetic and nondiabetic rats. Therefore, the developed formulation represents a potential drug delivery system for the effective incorporation of p-CA in wound healing therapy