Sequence analysis of a fifth segment of Penicillium rubens chrysovirus 1

In the present study, a Pakistani isolate of a known chrysovirus was identified in Penicillium rubens strain S25. This virus was found to have five genomic segments, ranging from 0.5 to 3.7 kbp in length. Analysis of the RNA-dependent RNA polymerase (RdRP) amino acid sequence revealed a 99.28% similarity with the genome of Penicillium chrysogenum chrysovirus 1, suggesting a close evolutionary relationship. Notably, a fifth segment, absent in P. chrysogenum , was identified and is described here for the first time in P. rubens . Sequence analysis showed that this fifth segment is 514 bp in length and has a 5'-terminus identical to the other four segments.