Isolation and Molecular characterization of a Novel Bacteriophage SVV09-A: Targeted to Staphylococcus ureilyticus from Diabetic Wounds

The pathogenic strains of Staphylococcus ureilyticus are one of the causes of frequently associated nosocomial infections in the hospital environment. The increasing antibiotic resistance in CoNS frequently results in treatment failures, highlighting the pressing requirement for new eradication methods. The present study mainly focused on isolation, and physiological characterization of phages from sewage water that target Staphylococcus ureilyticus , along with molecular characterization (whole genome sequencing) of phages and insilico analysis of its endolysin, including phylogenetic studies, open reading frames, and 3D model. Using the double-layer agar method, several phages were isolated, and the phage that has exhibited the broadest host range was selected. The current study reveals the genome of the lytic phage Staphylococcus ureilyticus , designated SVV09-A, which has been examined and annotated. Further analysis indicated that the phage has exhibited optimal activity at pH levels between 6 and 8 and within a temperature range of 30–37°C; manganese metal ions have shown great impact on phage adsorption rate. The whole genome sequence (WGS) of the phage SVV09-A was determined, revealing a linear DNA of 58,797 bp, with a G + C content of 46.9, and the phage was classified within the order Caudovirales. ORF analysis revealed 336 ORFs and uncovered functions for 67 genes. The estimated endolysin gene of phage SVV09-A had a length of 705 bp, which corresponds to 234 amino acids (~ 25.33 kDa). These findings offer a structural and functional basis for endolysin, establishing a framework for upcoming in vitro and in vivo efficacy research targeting multidrug-resistant Gram-positive infections.