Optimization of Growth Conditions for Arthrospira fusiformis in Laboratory and Outdoor Cultivation Settings

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Abstract

Arthrospira, with its rich essential nutrients like proteins, vitamins, fatty acids, minerals, and antioxidants, has drawn global attention as a potential food source and dietary supplement for humans . This study aimed to optimize the various growth parameters of Arthrospira fusiformis under laboratory and outdoor conditions. In the laboratory experiment, the growth of A. fusiformis was assessed at different pH levels (8.5, 9.0, 9.5, 10.0, 10.5, and 11.0), while maintaining a constant Dissolved Inorganic Carbon (DIC) level of 0.23 mol L -1 . Following this experiment, the growth of A. fusiformis was evaluated at different DIC levels (0.02, 0.09, 0.16, 0.23, and 0.30 mol L -1 ) with the optimum pH value of 10.0. Furthermore, an additional experiment was conducted outdoors in a raceway pond measuring 3.5 meters long, 1.9 meters wide, and 0.2 meters deep. The highest growth rate of A. fusiformis has been recorded at pH 10 [μ=1.324 ± 0.081 day -1 ], and the optimal DIC concentration was 0.23 mol L -1 . However, despite the highest growth rate being recorded at pH 10, the biggest dry weight (3.400 ± 0.015g L -1 ) and productivity (0.128 ± 0.001 g L -1 day -1) have been observed at the pH value of 11. Regarding the influence of DIC on the biomass of A. fusiformis , the highest dry weight was achieved at a DIC concentration of 0.3 mol L -1 (3.355 ± 0.010 g L -1 ), while the highest productivity was attained at a DIC concentration of 0.23 mol L -1 (0.127 ± 0.000 g L -1 day -1 ) with an OD750 measurement. Both pond temperature and pH of the pond water have affected the growth of A. fusiformis significantly (p < 0.05).

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