Influence of Adenine Sulphate on Growth Traits of Telfairia Occidentalis Under Tissue Culture Conditions

Optimization of the protocol in tissue culture for successful micro-propagation of Telfairia occidentalis (fluted pumpkin) is very important. Telfairia occidentalis is an important leafy vegetable in West Africa, particularly noted for its nutritional and economic benefits. This study looks into how adenine sulphate (AdS) affects the growth parameters of tissue-cultured Telfairia occidentalis in an effort to increase the effectiveness of in-vitro propagation. In this experiment, different concentrations of AdS (0, 25, 50, 75, 100) mg/ml were added to the culture media to ascertain its effects on some key growth parameters, including shoot length, root development and leaf proliferation. The study reveals that the relationship between adenine sulphate concentration and various growth parameters in Telfairia occidentalis exhibits non-linear trends, with U-shaped patterns. Maximum shoot induction and leaf number was observed in the control with zero AdS concentration while leaf length gave optimal value at concentration of 100mg/ml. Root length and the number of roots exhibit a U-shaped response, with growth declining at lower concentrations (around 50 mg/ml) before increasing again at higher concentrations. Similarly, vine/shoot length increases up to 40–50 mg/ml before decreasing sharply thus showing optimal values at 50mg/ml concentration. These results suggest that specific concentrations of AdS can either promote or inhibit growth depending on the parameter of interest. This study provides detailed insights into the dose-dependent effects of AdS on the growth of Telfairia occidentalis . It identifies specific concentration ranges that optimize leaf, root, and shoot growth, which can be used to enhance tissue culture protocols and improve propagation outcomes for this crop.