Characterization of grapevine endophytic microbes and field evaluation of bioformulations against powdery mildew disease with residue-compliant grape production
Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Grapevine ( Vitis vinifera L.) plays a major role in producing table grapes, raisins, juice, and wine in the global market. In the present study, 13 endophytes were isolated, purified, and identified by sequencing the ITS and 16S rRNA regions. Based on ITS sequence information, the fungal endophytes viz. , CSBY-2, CSBY-8, and MCBY-2, were identified as Trichoderma asperellum , whereas CSBY-4, MCBY-1, and DRRS-1 were identified as T. asperelloides , Cytospora sp., and T. viride , respectively. Similarly, the bacterial endophytes viz ., Sauvignon Blanc-2, -5, CS2, and RF1, were characterized as Bacillus subtilis . The RT7 and RB-1 were recognized as B. mojavensis and B. licheniformis , respectively, by sequencing 16S rRNA. The fungal endophytes were formulated into powder using flattened rice as a carrier, and bacterial isolates were used as liquid formulations. Bioformulations of these isolates were evaluated against grapevine powdery mildew disease during the 2022-23 and 2023-24 crop seasons at the experimental fields at ICAR-NRCG, Pune. In field evaluation of endophytic Trichoderma , Bacillus and Cytospora species formulations during the 2022-23 and 2023-24 crop seasons, the highest percent disease control (PDC) on leaves was recorded in treatment, Bacillus subtilis (RF-1: 63.41%) followed by B. subtilis (SB2: 61.57% and 58.11%), Bacillus sp. (GB2: 61.04%), B. subtilis (SB5: 59.66% and 56.65%), and Trichoderma asperellum (MCBY2: 51.07% and 60.93%). The highest PDC on bunches was observed in SB2 (64.56% and 44.97%), SB5: (56.18% and 46.07%), and MCBY-2: 55.88% and 67.7%) isolates. Moreover, all the fungal and bacterial formulations reduced the fungicide residues in the grape berries. The study provides important insights into managing grapevine diseases sustainably and suggests integrating endophytic microbes into traditional farming practices to enhance grape quality and productivity.
