Analysis of the Endophytic Fungi Community Structure and Function of Stephania tetrandra Based on High-throughput Sequencing

Analyzing the endophytic fungi in various Stephania tetrandra tissues is crucial for investigating strain resources, enhancing S. tetrandra cultivation, and carrying out further studies. In order to investigate the variety, community structure, and function of endophytic fungus in various tissues, the three distinct tissues of S. tetrandra 's roots, stem, and leaves were sequenced and analyzed using Illumina MiSeq high-throughput sequencing technology. Findings: 539 OTUs and 212,783 valid sequences throughout 11 phyla, 29 classes, 64 orders, 135 families, 222 genera, and 286 species were found. The major phylum and genus were Ascomycota and Cladosporium . Eight functional ecological groups were found in the endophytic fungi of S. tetrandra , according to the FUNGuild software analysis. The undefined functional groups made up a comparatively large percentage of the various tissues of S. tetrandra , which required more research. This work established a foundation for the development and use of S. tetrandra resources by revealing the diversity and structure of endophytic fungi in the roots, stems, and leaves of various S. tetrandra tissues.