Evaluation of antimicrobial adsorption capacity in blood culture medium

Objective: To evaluate the adsorption capacity of Merieux BacT/ALERT (FA Plus and FN Plus) bottles and HAPYEAR (aerobic and anaerobic) bottles for antimicrobial agents. Method : In simulated adult blood cultures, microbial-antimicrobial combinations were added to compare the inactivation capacity of commercial blood culture medium. The antibiotics used were imipenem, cefoxitin, vancomycin, cefoperazone/sulbactam, piperacillin/tazobactam, penicillin and micafungine. Result: The Merieux blood culture vial (bacteria + antibiotics) experimental group was all positive and had adsorption capacity for the antibiotics used in the experiment.However, the HAPYEAR results were partially positive, which only had adsorption capacity for piperacillin/tazobactam and micafungine, and had no adsorption capacity for most other antibiotics. In terms of the time to report positive results, the Merieux blood culture vial group (bacteria + antibiotics) was reported earlier. Among them, the average time of Merieux blood culture vial in the (Pseudomonas aeruginosa + piperacillin/Tazobactam) group was 8.4 hours earlier than that in the HAPYEAR group. The average time of the Merieux blood culture vial in the (Candida albicans + Micafunzin) group was 64.8 hours earlier than that in the HAPYEAR group. Conclusion: BacT/ALERT (FA Plus and FN Plus) culture medium have a relative advantage, but not an absolute advantage. Its detection rate is high, TAT is short, it can be selectively applied to patients who have used antibiotics in the past, and then take the blood culture samples.