Detection of Carbendazim with SA-AgNPs-CD functionalized silver nanoparticles as substrates based on Surface-Enhanced Raman Scattering

A novel surface-enhanced Raman scattering (SERS) substrate was developed to determination of the pesticide carbendazim (CBZ) through the green synthesis of silver nanoparticles (SA-AgNPs-CD) using sodium alginate as a reducing agent and β-cyclodextrin as a molecular recognition stabilizer. The composite material was systematically characterized by transmission electron microscopy (TEM) and UV-vis spectroscopy, revealing spherical nanoparticles (15-25 nm diameter) with localized surface plasmon resonance at 412 nm. 4-aminothiophenol (PATP) was used as a SERS probe to discuss the SERS activity of the substrate, Multivariate optimization of detection parameters (substrate-analyte ratio 3:1, pH 6.8, 15 min adsorption, sequential reagent addition) via orthogonal experimental design. The linear response extends from 5.0 × 10 ^-5 to 1.0 × 10 ^-7 mol/L for CBZ,combined with principal component analysis (PCA) revealed an ideal linear relationship between CBZ concentration and principal component F1, achieving a limit of detection(LOD) 1.95×10 ^-9 mol/L. It is expected that this SERS strategy will provide great value for rapid detecting pesticide residues in fruits and food products.