DNA cleavage activity of linear trinuclear copper(II) complexes
Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Metal ions and metal complexes are important components of nucleic acid biochemistry, participating both in the regulation of gene expression and as therapeutic agents. Cu(II) complexes are important for DNA cleavage, which is essential for the development of anticancer drugs and chemotherapeutic agents. For example, a study revealed that a Cu(II) complex caused double-strand DNA nicks, destabilized the DNA molecule, and disrupted the phosphodiester bonds. Here two trinuclear copper (II) complexes ware synthesized and structurally characterized: [Cu II 3 ( L 2 ) 4 (2picolinate) 2 (OClO 3 ) 2 ](ClO 4 ) 2 •2CH 3 COCH 3 •2CH 3 OH ( 1 ) and [Cu II 3 ( L 2 ) 4 (2,6-dipicolinate) 2 ] (ClO 4 ) 2 •6MeOH•2H 2 O ( 2 ) ( L 2 = 1-benzyl-[3-(2'-pyridyl)] pyrazole; 2-picolinate = 2-pyridinecarboxylic acid; 2,6-dipicolinate = 2,6-pyridinedicarboxylic acid). The binding of copper complexes to plasmid DNA (P-DNA) has been investigated via UV-Visible spectroscopy, which revealed that P-DNA can covalently bind to the complexes. P-DNA cleavage was also investigated via agarose gel electrophoresis in the presence and absence of an oxidative agent (H 2 O 2 ). The effect of the complex concentration on the P-DNA cleavage reaction has also been studied. Both copper complexes show nuclease activity, which significantly depends on the concentrations of the complexes, in the presence of H 2 O 2, likely through an oxidative mechanism whereas they slightly cleave P-DNA in the absence of an oxidative agent.
