Assembly of the complete mitochondrial genome of Prinsepia uniflora (Rosaceae): Insights into genomic characterization and phylogenetic analysis

Background Prinsepia uniflora is a significant medicinal and ecological value shrub. Although the genome of this species has been sequenced at the chromosome scale, reports on the mitogenomes of Prinsepia plants are lacking, substantially impedes further research on this species. Results We used PacBio HiFi sequencing to assemble the mitogenome of P. uniflora . The mitogenome was characterized is a circular chromosome, with a total length of 328,095 bp; the genes included 35 protein-coding genes, 18 transfer RNA (tRNA) genes and 3 ribosomal RNA (rRNA) genes. Further, our mitogenome characteristics investigation of the P. uniflora included codon usage, sequences repeats and selective pressure. The results showed that a large number of short repetitive sequences existed in the mitogenome of P. uniflora , the codon usage showed a preference for codons ending in A/T, and the codon use bias of the PCGs was modified by natural orartificial selection. We also identifed 20 large fragments that were transferred from the chloroplast genome to the mitogenome. The collinear analysis of mitochondrial structures between P. uniflora and Rosaceae species indicated that P. uniflora was closely related to Prunus padus . Phylogenetic examination based on ML and BI methods also revealed the evolutionary status of the P. uniflora . Conclusion In this study, we assembled and annotated the first mitogenome of the Prinsepia . The subsequent analysis provided us with a basis for further molecular studies on Prinsepia mitogenomes and valuable information on the evolutionary biology of Rosaceae species.