Potential exploration of silver nitrate nanoparticlesloaded on Conyzacanadensis forin vitro and in vivocytotoxic and immunologicalstudies

This study aimed to evaluate the biological effects of C. canadensis extract and its silver nanoparticles, focusing on their cytotoxic and anti-inflammatory properties by assessing their effectiveness in cytokine production and wound healing potential in albino male mice. Our research revealed that C. canadensis extracts are rich in flavonoids, phenols, and saponins. Wound healing results indicated that burns treated with an aqueous extract of C. canadensis healed in 24 days, compared to 18 days for those treated with silver nanoparticles. Notably, mice treated with green synthetic nanoparticles recovered after just 13 days. The study also demonstrated the anti-inflammatory effects of C. canadensis extract and biosynthesized silver nanoparticles on the in vitro release of cytokines (IL-6 and IL-10) from cultured mononuclear cells. These results highlight the potential of C. canadensis as a valuable source of bioactive compounds for developing new drugs, cosmetics, and food products across various industrial sectors. Silver nanoparticles and C. canadensis extracts could be effectively used as wound healing and anti-inflammatory has been performed for the first time in these species.