Circumvention of Hepato-toxicity of Paracetamol by Co-administering with Flavonoids in the treatment of Osteoarthritis

Objectives The objective of present study is to develop novel pharmaceutical dosage form to reduce the hepatotoxicity of Paracetamol when induced in higher doses in treatment of Osteoarthritis. The effect of Quercetin in controlling paracetamol toxicity was planned to be studied by conducting in vivo studies on rats for 21days ¹ . The outcome of the present study is to show that Quercetin shows high impact in reducing the hepatotoxicity caused by paracetamol due to release of toxic metabolite NAPQI by cytochrome P-450 during metabolism process. Materials and methods Rats were treated with Paracetamol in combination with different dose strengths of quercetin, and with silymarin as standard. Results Quercetin is given in doses of 5, 10, and 15 mg/kg body weight. with paracetamol lowered the blood levels of SGOT, SGPT, ALP, DB, and TB while restoring albumin & total protein levels to normal in a dose-varying way in comparison to the toxic control. Histopathology of liver and kidney were done for cell necrosis ² . Chrsyin therapy at low doses resulted in a modest recovery from necrosis, vacuolization, inflammation, and fatty alterations. Moderate recovery from necrosis, vacuolization, lipid alterations, and inflammation was observed with medium dose therapy ³ . Treatment at a high dose maintained the liver's natural architecture. Conclusion The current study found that Quercetin inhibits the CYP2E1 mediated metabolism, as a result reduces the formation of toxic metabolite NAPQI by suppressing the liver and kidney biomarkers ⁴ . Hence reducing the liver toxicity