Roseateles cavernae sp. nov. ， a bacterium isolated from freshwater of the Old Huanglong Cave in Yunnan province, south-west China

A Gram-stain-negative, rod-shaped, amylolytic bacterial strain, designated as YIM B04394T, was isolated from a freshwater sample collected from the Old Huanglong Cave in Kunming, Yunnan province, south-west China. The strain was found to be aerobic, oxidase-positive and weakly catalase- positive, grew at 10–40℃ (optimum, 28°C), pH 6.0–8.0 (optimum, pH 7.0) and low NaCl concentration (up to 5.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM B04394T clustered with Roseateles species. The DNA-DNA and ANI relatedness values of strain YIM B04394 T with the related species R. toxinivorans DSM 16998T and R. asaccharophilus DSM 25082T were 21.50-22.9%% and 76.47-76.93%, respectively. Major fatty acids included summed feature 3 and C16:0. MK-8 was detected as the respiratory quinone and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The DNA G + C content was 67.89 mol%. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations indicated that the strain represents a novel Roseateles species for which the name Roseateles cavernae sp. nov. is proposed. The type strain is YIM B04394T (= NBRC 115775T = KCTC 92438T = CCTCC AB2021502T).