Rapid and convenient detection of Weissella ceti with loop-mediated isothermal amplification

Weissella ceti , one of Gram-positive lactic‐acid bacteria, as a newly emerging pathogen can cause the rainbow trout ( Oncorhynchus mykiss ) with Weissellosis. Until now, it is not easy to identify W. ceti from other pathogens by symptoms of the fish infected especially at the primary stage of infection, and thus a method of identification was needed to determine conveniently and quickly this pathogen. In this study, loop-mediated isothermal amplification (LAMP) detection has higher specificity and sensitivity for W. ceti . The primers according to the hemolysin A gene were designed, and the LAMP detection was accomplished within 30 min at 63 ℃. The mimimum concentration of LAMP detection for W. ceti was 5.9*10 ^− 6 ng/µl, which was 100 times more sensitive than traditional PCR. The LAMP detection confirmed the high specificity for detecting W. ceti , whereas the other six pathogens used were all negative reactions. In addition, it was validated that the LAMP detection was also effective in simulated clinical samples. Based on the results, the LAMP detection was rapid and convenient for point-of-care testing diagnosis of W. ceti .