Polydiacetylene Based Bio Sensing Strategy for the Detection of Ndv and Csf Antibodies for Diva Strategy

Influenza viruses have become a major threat to humans, animals and agriculture posing a huge serious economic loss. In this connection, a rapid, high sensitive and cost effective detection method is the need of the day to keep a regular surveillance and to control the spreading of the viruses. Newcastle disease virus (NDV) is most prevalent virus in avian species causing disastrous losses in worldwide poultry industries. Despite of the availability of several vaccines, eradication of the disease still remains a challenge due to the inability of the conventional vaccines to differentiate the infected from vaccinated animals (DIVA).This led to the need for the development of marker vaccines which in conjunction with diagnostic tools that are able to differentiate the infected animals from the vaccinated animals. The limitations possessed by conventional diagnostic tools has paved the way to the implementation of biosensors for its rapid, cost effective, on site detecting features. This research work advocates, the development of a bio sensing strategy for the detection of anti-bodies developed when the chickens exposed to Newcastle disease virus (NDV) and Classical Swine Fever (CSF) using peptide-functionalized polydiacetylene (PEP-PDA) based on the unique color variation (bio-chromism) of the PDA. The PDA vesicle is formed from the ratio of 10, 12-pentacosadionic acid (PCDA), 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) using a peptide of NDV and CSF attached to the surface of the vesicle. The specificity, sensitivity and proficiency of the method is evaluated. The developed method is very cost effective, advantageous than existing methods.