SARS‐CoV‐2 Alpha, Beta, and Delta variants display enhanced Spike‐mediated syncytia formation
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
-
SciScore for 10.1101/2021.06.11.448011: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Informed consent was provided by the individuals for use of their biological materials. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Contamination: All cells lines were either purchased from ATCC or were kind donations from members of the Institut Pasteur and were routinely screened for mycoplasma. Table 2: Resources
Antibodies Sentences Resources Antibody binding to S proteins was assessed via s analogous protocol where transfected 293T cells were first stained with either human mAb10 (pan-coronavirus anti-S2), mAb102 and mAB129 (pan-SARS-CoV-2), mAb48 and mAb98 (SARS-CoV-2 anti-RBD), and mAb71 … SciScore for 10.1101/2021.06.11.448011: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Informed consent was provided by the individuals for use of their biological materials. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Contamination: All cells lines were either purchased from ATCC or were kind donations from members of the Institut Pasteur and were routinely screened for mycoplasma. Table 2: Resources
Antibodies Sentences Resources Antibody binding to S proteins was assessed via s analogous protocol where transfected 293T cells were first stained with either human mAb10 (pan-coronavirus anti-S2), mAb102 and mAB129 (pan-SARS-CoV-2), mAb48 and mAb98 (SARS-CoV-2 anti-RBD), and mAb71 (SARS-CoV-2 anti-NTD) at 1 µg/mL. anti-S2suggested: Noneanti-RBDsuggested: Noneanti-NTDsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cells: Vero E6, HEK293T, U20S, Caco2/TC7, Calu3 were cultured in DMEM with 10% Fetal Bovine Serum (FBS) and 1% Penicillin/Streptomycin (PS). HEK293Tsuggested: NoneVero and 293T GFP-split cells transduced cells with pQCXIP were cultured with 4ug/ml and 1 ug/ml of puromycin (InvivoGen), respectively. Verosuggested: NoneAntibody binding to S proteins was assessed via s analogous protocol where transfected 293T cells were first stained with either human mAb10 (pan-coronavirus anti-S2), mAb102 and mAB129 (pan-SARS-CoV-2), mAb48 and mAb98 (SARS-CoV-2 anti-RBD), and mAb71 (SARS-CoV-2 anti-NTD) at 1 µg/mL. 293Tsuggested: NoneRecombinant DNA Sentences Resources Plasmids: A codon optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank: AJ318514), by replacing the VSV-G gene. phCMVsuggested: RRID:Addgene_15802)pQCXIP-Empty control plasmid, pQCXIP-IFITM1-N-FLAG, pQCXIP-IFITM2-N-FLAG, pQCXIP-IFITM3-N-FLAG were previously described (Buchrieser et al, 2019). pQCXIP-BSR-GFP11 and pQCXIP-GFP1-10 were from Yutaka Hata ((Kodaka et al, 2015); Addgene plasmid #68716; http://n2t.net/addgene:68716; RRID: Addgene_68716 and Addgene plasmid #68715; http://n2t.net/addgene:68715; RRID: Addgene_68715). pQCXIP-Emptysuggested: NonepQCXIP-IFITM1-N-FLAGsuggested: NonepQCXIP-IFITM2-N-FLAGsuggested: NonepQCXIP-IFITM3-N-FLAGsuggested: Nonedetected: RRID:Addgene_68716)detected: RRID:Addgene_68715)pcDNA3.1-hACE2 was from Hyeryun Choe ((Li et al, 2003); Addgene plasmid # 1786; http://n2t.net/addgene:1786; RRID: Addgene_1786). pcDNA3.1-hACE2suggested: RRID:Addgene_145033)detected: RRID:Addgene_1786)pCSDest-TMPRSS2 was from Roger Reeves ((Edie et al, 2018); Addgene plasmid # 53887; http://n2t.net/addgene:53887; RRID: Addgene_53887). pCSDest-TMPRSS2suggested: Nonedetected: RRID:Addgene_53887)Vero and 293T GFP-split cells transduced cells with pQCXIP were cultured with 4ug/ml and 1 ug/ml of puromycin (InvivoGen), respectively. pQCXIPsuggested: RRID:Addgene_15714)10 ng of phCMV-SARS-CoV2-S and/or 25 ng of pCDNA3.1-hACE2, 25 ng of pCSDest-TMPRSS2, and 40 ng of pQCXIP-IFITM were used and adjusted to 100 ng DNA with pQCXIP-Empty. pQCXIP-IFITMsuggested: NoneSoftware and Algorithms Sentences Resources Fusion defined as percent of GFP pixels was calculated with ImageJ. ImageJsuggested: (ImageJ, RRID:SCR_003070)Statistical analysis: Flow cytometry data was analyzed with FlowJo v10 software (Tristar). FlowJosuggested: (FlowJo, RRID:SCR_008520)Calculations were all performed with Microsoft Excel 365. Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)GraphPad Prism 9 was used to generate figure and for statistical analysis. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 56, 51 and 54. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-