Pooled RNA sample reverse transcriptase real time PCR assay for SARS CoV-2 infection: A reliable, faster and economical method
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Abstract
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SciScore for 10.1101/2020.04.25.20079095: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: ETHICAL APPROVAL: The study was approved by the Institutional ethics committee and patient consent form was waived off because of the use of deidentified discarded RNA samples.
Consent: ETHICAL APPROVAL: The study was approved by the Institutional ethics committee and patient consent form was waived off because of the use of deidentified discarded RNA samples.Randomization POOLING OF SAMPLES FOR THE RT-qPCR: RNA samples that were obtained after extraction were randomly pooled initially in pools of 2, 4, 6, 8, 16 RNA elutes on a 96 well plate. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key …
SciScore for 10.1101/2020.04.25.20079095: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: ETHICAL APPROVAL: The study was approved by the Institutional ethics committee and patient consent form was waived off because of the use of deidentified discarded RNA samples.
Consent: ETHICAL APPROVAL: The study was approved by the Institutional ethics committee and patient consent form was waived off because of the use of deidentified discarded RNA samples.Randomization POOLING OF SAMPLES FOR THE RT-qPCR: RNA samples that were obtained after extraction were randomly pooled initially in pools of 2, 4, 6, 8, 16 RNA elutes on a 96 well plate. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Pooling can also be done by pooling the VTMs and doing common extraction for pooled VTM samples, here the limitation is that in case a pool is positive individual sample collection has to be done at the patient level. Pooling RNA samples are easy to do in a laboratory as any pool turns out to be positive ID test can be performed and result can be declared. It can be argued that the sample size of our study is small. However, in the interest of widespread need to enhance testing facilities and capabilities, the present study makes a seminal contribution. In future, mathematical and computational model can be applied to design appropriate pool size and increase the pool size further for SARS-CoV-2 testing. Pooling of samples is essentially important in monitoring the infection in cohesive groups such as quarantine facilities, health care workers, community surveillance and diagnosing the asymptomatic cases. The infection load in these groups may be low but even a single positive case amongst such groups can activate the surveillance system and quarantine the affected group and prevent the further spread in the community.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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