Prevalence of SARS-Cov-2 antibodies and living conditions: the French national random population-based EPICOV cohort
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Abstract
Background
We aimed to estimate the seroprevalence of SARS-CoV-2 infection in France and to identify the populations most exposed during the first epidemic wave.
Methods
Random selection of individuals aged 15 years or over, from the national tax register (96% coverage). Socio-economic data, migration history, and living conditions were collected via self-computer-assisted-web or computer-assisted-telephone interviews. Home self-sampling was performed for a random subsample, to detect IgG antibodies against spike protein (Euroimmun), and neutralizing antibodies with in-house assays, in dried blood spots (DBS).
Results
The questionnaire was completed by 134,391 participants from May 2nd to June 2st, 2020, including 17,441 eligible for DBS 12,114 of whom were tested. ELISA-S seroprevalence was 4.5% [95% CI 3.9–5.0] overall, reaching up to 10% in the two most affected areas. High-density residences, larger household size, having reported a suspected COVID-19 case in the household, working in healthcare, being of intermediate age and non-daily tobacco smoking were independently associated with seropositivity, whereas living with children or adolescents did not remain associated after adjustment for household size. Adjustment for both residential density and household size accounted for much of the higher seroprevalence in immigrants born outside Europe, twice that in French natives in univariate analysis.
Conclusion
The EPICOV cohort is one of the largest national representative population-based seroprevalence surveys for COVID-19. It shows the major role of contextual living conditions in the initial spread of COVID-19 in France, during which the availability of masks and virological tests was limited.
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SciScore for 10.1101/2021.02.24.21252316: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Antibodies Sentences Resources All samples with an ELISA-S test optical density ratio ≥ 0.7 were also tested with an in-house micro-neutralization assay to detect neutralizing anti-SARS-CoV-2 antibodies (SN) (15). anti-SARS-CoV-2suggested: NoneExperimental Models: Cell Lines Sentences Resources For these tests, VeroE6 cells cultured in 96-well microplates, 100 TCID50 of SARS-CoV-2 strain BavPat1 (courtesy of Prof. Drosten, Berlin, Germany) and serial dilutions of serum (1/20–1/160) were used. VeroE6suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)Results from OddPub: We did not detect open data. We also did not detect open code. …
SciScore for 10.1101/2021.02.24.21252316: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Antibodies Sentences Resources All samples with an ELISA-S test optical density ratio ≥ 0.7 were also tested with an in-house micro-neutralization assay to detect neutralizing anti-SARS-CoV-2 antibodies (SN) (15). anti-SARS-CoV-2suggested: NoneExperimental Models: Cell Lines Sentences Resources For these tests, VeroE6 cells cultured in 96-well microplates, 100 TCID50 of SARS-CoV-2 strain BavPat1 (courtesy of Prof. Drosten, Berlin, Germany) and serial dilutions of serum (1/20–1/160) were used. VeroE6suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Limitations We did not included people living in residences for elderly. It was not feasible during the first lockdown. Specific studies will be required to provide reliable estimates for these populations, greatly affected by the epidemic. In recent decades, willingness to participate in population-based surveys has decreased considerably worldwide (36,37). We anticipated an overall response rate at about 50% in mainland France, including 70% of initial web or telephone contact, among them 70% of completed questionnaires. This took into account the difficulties to reach the selected individual in a household as explained above, and relocations and deaths since 2018- last updated date of the Fideli sampling frame. Calculation for the sample selection was performed considering multimodal web/telephone data collection for the whole sample. However, due to the limited number of simultaneous telephone interviewers during lockdown, we decided to offer multimodal interview for only four of the 20 random subsample lots (extended to five in the French West Indies and nine on Reunion Island), giving an overall response rate of 36%. It should be stressed that the response rate for the multimodal lots was close to that expected: 45% for the three concurrent mixed-mode subsamples and 44% for the sequential mixed-mode subsample. The CATI response rate reached 46% in concurrent multimodal administration, but did not exceed 29% for sequential multimodal administration, confirming that the s...
Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04378582 Completed Characteristics and Outcomes of Patients With COVID-19 Admit… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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