Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of Concern
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Abstract
During the coronavirus disease 2019 (COVID-19) pandemic, several variants of concern (VOCs) have been detected, for example, B.1.1.7, B.1.351, P.1, and B.1.617.2. The VOCs pose a threat to public health efforts to control the spread of the virus.
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SciScore for 10.1101/2021.06.23.21258782: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Ethical Statement: The PHO Ethics Review Board has determined that this project did not require research ethics committee approval, as it describes analyses that were completed at PHO Laboratory as part of routine clinical respiratory testing and surveillance during the COVID-19 pandemic in Ontario and are, therefore, considered public health practice and exempt from this requirement. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources In addition, a subset of all SARS-CoV-2 positive specimens underwent WGS as part of the provincial genomic surveillance program, … SciScore for 10.1101/2021.06.23.21258782: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Ethical Statement: The PHO Ethics Review Board has determined that this project did not require research ethics committee approval, as it describes analyses that were completed at PHO Laboratory as part of routine clinical respiratory testing and surveillance during the COVID-19 pandemic in Ontario and are, therefore, considered public health practice and exempt from this requirement. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources In addition, a subset of all SARS-CoV-2 positive specimens underwent WGS as part of the provincial genomic surveillance program, which included VOC screening. WGSsuggested: NoneIn addition, a BLAST search of the primer and probe sequences against the whole genome of the reference sequence (MN908947.3) was conducted to ensure the absence of homology to other parts of the SARS-CoV-2 genome. BLASTsuggested: (BLASTX, RRID:SCR_001653)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a protocol registration statement.
Results from scite Reference Check: We found no unreliable references.
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