Impact of Prior Infection on SARS-CoV-2 Antibody Responses in Vaccinated Long-Term Care Facility Staff

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1. Version published to 10.1128/msphere.00169-22

   Aug 31, 2022
2. 

   ScreenIT

   Apr 8, 2022

   SciScore for 10.1101/2022.04.04.487083: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: Human specimens: This study was approved by the Colorado State University Institutional Review Board under protocol number 20-10057H.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Plates were washed and a secondary anti-human IgG-horseradish peroxidase conjugated secondary antibody was added.	anti-human IgG-horseradish suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, diluted sera were mixed with virus, incubated for one hour at 37°C, added to a Vero cell monolayer, incubated an additional hour at 37°C, then overlaid …

   SciScore for 10.1101/2022.04.04.487083: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: Human specimens: This study was approved by the Colorado State University Institutional Review Board under protocol number 20-10057H.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Plates were washed and a secondary anti-human IgG-horseradish peroxidase conjugated secondary antibody was added.	anti-human IgG-horseradish suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, diluted sera were mixed with virus, incubated for one hour at 37°C, added to a Vero cell monolayer, incubated an additional hour at 37°C, then overlaid with tragacanth media and incubated for two days.	Vero suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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3. 

   Version published to 10.1101/2022.04.04.487083v1 on bioRxiv

   Apr 5, 2022