Highly Sensitive and Specific Multiplex Antibody Assays To Quantify Immunoglobulins M, A, and G against SARS-CoV-2 Antigens

  1. Carlota Dobaño
  2. Marta Vidal
  3. Rebeca Santano
  4. Alfons Jiménez
  5. Jordi Chi
  6. Diana Barrios
  7. Gemma Ruiz-Olalla
  8. Natalia Rodrigo Melero
  9. Carlo Carolis
  10. Daniel Parras
  11. Pau Serra
  12. Paula Martínez de Aguirre
  13. Francisco Carmona-Torre
  14. Gabriel Reina
  15. Pere Santamaria
  16. Alfredo Mayor
  17. Alberto L. García-Basteiro
  18. Luis Izquierdo
  19. Ruth Aguilar
  20. Gemma Moncunill

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Abstract

Reliable serological tests are required to determine the prevalence of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to characterize immunity to the disease in order to address key knowledge gaps in the coronavirus disease 2019 (COVID-19) pandemic. Quantitative suspension array technology (qSAT) assays based on the xMAP Luminex platform overcome the limitations of rapid diagnostic tests and enzyme-linked immunosorbent assays (ELISAs) with their higher precision, dynamic range, throughput, miniaturization, cost-efficiency, and multiplexing capacity.

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  1. Version published to 10.1128/jcm.01731-20
  2. ScreenIT

    SciScore for 10.1101/2020.06.11.147363: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementConsent: Samples analyzed in this study received ethical clearance for immunological evaluation and/or inclusion as controls in immunoassays, and the protocols and informed consent forms were approved by the Institutional Review Board (IRB) at HCB (Refs.
    IRB: Samples analyzed in this study received ethical clearance for immunological evaluation and/or inclusion as controls in immunoassays, and the protocols and informed consent forms were approved by the Institutional Review Board (IRB) at HCB (Refs.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Antigen-coupled beads were validated by incubating them with serial dilutions of anti-histidine-Biotin antibody for antigens with a histidine-tag (Abcam, ab27025).
    anti-histidine-Biotin
    suggested: None
    For the first option, 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140,
    anti-human IgG
    suggested: (Sigma-Aldrich Cat# B1140, RRID:AB_258513)
    Seropositivity threshold (cutoff) for optimization tests was calculated as 10 to the mean plus 3 standard deviations of log10-transformed MFIs of the negative controls for each antibody isotype and antigen.
    antigen.
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.06.11.147363v1 on bioRxiv