Large-Scale, In-House Production of Viral Transport Media To Support SARS-CoV-2 PCR Testing in a Multihospital Health Care Network during the COVID-19 Pandemic
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Abstract
The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription-PCR (RT-PCR) testing. In response to this ongoing shortage, we began production of VTM in-house in support of diagnostic testing in our hospital network. As our diagnostic laboratory was not equipped for reagent production, we took advantage of space and personnel that became available due to closure of the research division of our medical center.
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SciScore for 10.1101/2020.04.29.20085514: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization Next, one ml of media from a randomly selected tube in each lot was also plated on chocolate agar. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Finally, a randomly selected tube of media from each lot was tested for its support of SARS-CoV-2 RT-PCR testing using the EUA-authorized Abbott RealTime SARS-CoV-2 assay run on the Abbott m2000 platform, the same system used for clinical testing in our healthcare network. Abbottsuggested: (Abbott, RRID:SCR_010477)All spiked VTM Ct values were recorded and visualized in aggregate as a … SciScore for 10.1101/2020.04.29.20085514: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization Next, one ml of media from a randomly selected tube in each lot was also plated on chocolate agar. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Finally, a randomly selected tube of media from each lot was tested for its support of SARS-CoV-2 RT-PCR testing using the EUA-authorized Abbott RealTime SARS-CoV-2 assay run on the Abbott m2000 platform, the same system used for clinical testing in our healthcare network. Abbottsuggested: (Abbott, RRID:SCR_010477)All spiked VTM Ct values were recorded and visualized in aggregate as a Levy-Jennings plot using GraphPad Prism 7.0. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Through collaboration between researchers, clinical microbiologists, and support/logistics personnel, we were able to circumvent the limitations of a routine diagnostic laboratory and address a significant bottleneck that would otherwise limit high volume COVID-19 testing capacity.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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