Identification of Antiviral Drug Candidates against SARS-CoV-2 from FDA-Approved Drugs
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Abstract
Drug repositioning is the only feasible option to immediately address the COVID-19 global challenge. We screened a panel of 48 FDA-approved drugs against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which were preselected by an assay of SARS-CoV. We identified 24 potential antiviral drug candidates against SARS-CoV-2 infection. Some drug candidates showed very low 50% inhibitory concentrations (IC 50 s), and in particular, two FDA-approved drugs—niclosamide and ciclesonide—were notable in some respects.
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SciScore for 10.1101/2020.03.20.999730: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Anti-SARS-CoV-2 N protein antibody was purchased from Sino Biological Inc. (Beijing, China) Anti-SARS-CoV-2 N protein antibodysuggested: NoneAnti-SARS-CoV-2 N proteinsuggested: NoneAlexa Fluor 488 goat anti-rabbit IgG (H + L) secondary antibody and Hoechst 33342 were purchased from Molecular Probes. Alexa Fluor 488 goat anti-rabbit IgGsuggested: Noneanti-rabbit IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Vero cells were seeded at 1.2 … SciScore for 10.1101/2020.03.20.999730: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Anti-SARS-CoV-2 N protein antibody was purchased from Sino Biological Inc. (Beijing, China) Anti-SARS-CoV-2 N protein antibodysuggested: NoneAnti-SARS-CoV-2 N proteinsuggested: NoneAlexa Fluor 488 goat anti-rabbit IgG (H + L) secondary antibody and Hoechst 33342 were purchased from Molecular Probes. Alexa Fluor 488 goat anti-rabbit IgGsuggested: Noneanti-rabbit IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Vero cells were seeded at 1.2 × 104 cells per well in DMEM, supplemented with 2% FBS and 1X Antibiotic-Antimycotic solution (Gibco) in black, 384-well, μClear plates (Greiner Bio-One), 24 h prior to the experiment. Verosuggested: RRID:CVCL_ZW93)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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