Long-chain polyphosphates impair SARS-CoV-2 infection and replication
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Abstract
Long-chain polyphosphates inhibit SARS-CoV-2 infection by targeting a host receptor and a viral RNA polymerase.
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SciScore for 10.1101/2020.11.18.388413: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell culture: Vero cells were kept in DMEM (41966-029; Gibco) with 10% FBS (10270-106; Gibco), 2 mM L-glutamine (25030-024; Gibco), and 1% penicillin/streptomycin (P0781; Sigma) Verosuggested: NoneCaco2 cells were cultured in EMEM (M2279, Sigma-Aldrich) with 10% FBS (10270-106; Gibco), 2 mM L-glutamine (25030-024; Gibco), and 1% penicillin/streptomycin (P0781; Sigma). Caco2suggested: NoneFor proteasome inhibition treatment Vero E6 cells … SciScore for 10.1101/2020.11.18.388413: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell culture: Vero cells were kept in DMEM (41966-029; Gibco) with 10% FBS (10270-106; Gibco), 2 mM L-glutamine (25030-024; Gibco), and 1% penicillin/streptomycin (P0781; Sigma) Verosuggested: NoneCaco2 cells were cultured in EMEM (M2279, Sigma-Aldrich) with 10% FBS (10270-106; Gibco), 2 mM L-glutamine (25030-024; Gibco), and 1% penicillin/streptomycin (P0781; Sigma). Caco2suggested: NoneFor proteasome inhibition treatment Vero E6 cells were incubated in DMEM (10% FBS) containing 10uM Mg132 (M8699; Sigma-Aldrich) for 24h; for protein synthesis inhibitor treatment, Vero E6 cells were incubated in DMEM (10% FBS) containig 50 ug/mL cycloheximide (C7698; Sigma-Aldrich) for 24h. Vero E6suggested: NoneHEK-293T or Human primary nasal epithelial cells were transiently transfected with the plasmid DNA constructs (i.e. HA-ubiquitin plasmid, pGBW-m4134165 HEK-293Tsuggested: NoneSoftware and Algorithms Sentences Resources Hek 293T transfected cells were then treated for 24h with Polyp120 and harvested for Western blotting 48 h after transfection. Polyp120suggested: NoneImmunofluorescence data were analysed by two-sample t-tests using the SPSS software, version 16.0. SPSSsuggested: (SPSS, RRID:SCR_002865)Electrostatic potential and docking experiments: Electrostatic potential calculations were carried out with Adaptive Poisson-Boltzmann Solver software (41) within the AutoDock Tools environment (42) using the default parameters. AutoDocksuggested: (AutoDock, RRID:SCR_012746)Mapping of the electrostatic potential onto the protein surface and display of the molecules was carried out with PyMOL Molecular Graphics system (Version 1.8, Schrodinger LLC, 2015). PyMOLsuggested: (PyMOL, RRID:SCR_000305)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04292899 Completed Study to Evaluate the Safety and Antiviral Activity of Remde… NCT04292730 Completed Study to Evaluate the Safety and Antiviral Activity of Remde… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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