Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites
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Abstract
Building mammalian embryos from self-organizing stem cells in culture would accelerate the investigation of morphogenetic and differentiation processes that shape the body plan. Veenvliet et al. report a method for generating embryonic trunk-like structures (TLSs) with a neural tube, somites, and gut by embedding mouse embryonic stem cell aggregates in an extracellular matrix surrogate. Live imaging and comparative single-cell transcriptomics indicate that TLS formation is analogous to mouse development. TLSs therefore provide a scalable, tractable, and accessible high-throughput platform for decoding mammalian embryogenesis at a high level of resolution.
Science , this issue p. eaba4937
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Excerpt
What would happen if you culture in vitro self-aggregated gastruloids embedded in Matrigel? Believe it or not, they differentiate into trunk-like structures containing neural tube and somites.
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Excerpt
Creating to understand: mouse embryonic stem cells can be aggregated in specific conditions to mimic formation of embryonic precursors of spinal cord and body segments called somites
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