Rapid and Safe Neutralization Assay for Circulating H5N1 Influenza Virus in Dairy Cows
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Rapid and safe neutralization assays are required for highly pathogenic avian influenza viruses, including a clade 2.3.4.4b H5N1 subtype recently found in cows. Here, we report a neutralization assay using luminescent virus‐like particles. This assay has lower biosafety requirements and provides a larger dynamic range than conventional methods. We applied this technique to evaluate the cross‐reactivity of neutralizing antibodies induced by clade 2.3.4.4b candidate vaccine viruses (CVVs) with the cow‐derived H5N1 virus. Our findings indicate that these CVVs share antigenic characteristics with the cow‐derived H5N1 virus, suggesting the potential efficacy of vaccines developed using these CVVs.
