Engineering Spatial Control of Bacterial Organelles
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This article is not in any list yet, why not save it to one of your lists.Abstract
Bacteria were once thought to lack organelles, but it is now clear they confine cellular reactions using an array of membrane- and protein-based compartments. A central question, however, is how bacterial organelles are organized in the cell, and whether their spatial control can be engineered. Here, we show that a two-protein system (McdAB) that positions carboxysomes-CO 2 -fixing organelles found in autotrophic bacteria-can be repurposed to provide programmable spatial control to diverse organelles in Escherichia coli . McdAB not only restores proper assembly and positioning of heterologously expressed carboxysomes in E. coli , but can also be reprogrammed to spatially organize all other known types of bacterial organelles, including encapsulins, biomolecular condensates, and even membrane-bound organelles. Programmable spatial organization of bacterial organelles establishes a new design principle for synthetic biology, where the location of reactions is as tunable as their content. Our work paves the way for more efficient biocatalysis in engineered microbes.
