PLCγ2 Controls Neutrophil Sensitivity through Calcium Oscillation and Gates Chemoattractant Concentration Range for Chemotaxis

The connection between calcium oscillation and cell sensitivity is poorly understood. Calcium oscillation is triggered either spontaneously or upon receptor-ligand binding. The cytosolic [Ca ²⁺ ] increase during calcium oscillation is initiated from Ca ²⁺ release from the intracellular stores through the phospholipase C (PLC)-derived inositol 1,4,5-trisphosphate (IP ₃ ). Here, we show that neutrophils lacking PLCγ2 ( plcg2 ^kd ) display impaired spontaneous calcium oscillation and chemoattractant-induced calcium response, decreased membrane targeting of CAPRI (a RasGAP), and subsequent increased activations of Ras and its effectors, such as PI ₃ Kγ activation and actin polymerization. More importantly, plcg2 ^kd neutrophils sense and respond to chemoattractant at a subsensitive chemoattraction. Taken together, our results demonstrate that PLCγ2 mediates spontaneous calcium oscillation, contributes to chemoattractant-triggered calcium response, controls neutrophil sensitivity through membrane targeting of CAPRI, and gates chemoattractant concentration range for neutrophil chemotaxis.