A novel loop-mediated isothermal amplification (LAMP) diagnostic test for detecting Salmonella enterica serotype Typhi directly in raw milk
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Milk-transmitted outbreaks remain a significant issue in low-income countries due to unhygienic practices. There are no known easily accessible and cheap diagnostic tests that can detect S . Typhi in raw milk samples at high specificity, sensitivity, and speed without preprocessing. Using Loop-Mediated Isothermal Amplification (LAMP) we detected a previously identified target gene, STY2879, as low as 10 3 copies of DNA/μL in 25% diluted raw milk samples. The user-friendly LAMP system has a colorimetric readout and potential for use in remote and low-resource settings where S . Typhi is a problem.
