Promoter replacement by genome editing creates gain-of-function traits in Arabidopsis
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We introduced two targeted DNA double-strand breaks on the same Arabidopsis chromosome using CRISPR-Cas9 and replaced the FLOWERING LOCUS T ( FT ) promoter with that of a histone variant gene through chromosomal inversion. The resulting lines misexpressed FT and flowered early, like FT -overexpressing transgenic plants. This system can be used to create gain-of-function mutations that modify target gene expression as desired without incorporating foreign DNA sequences.
