Sub-chronic and acute toxicity of 6PPD-quinone to early-life stage rainbow trout ( Oncorhynchus mykiss )

N-(1,3-Dimethylbutyl)-N’-phenyl-p-phenylenediamine-quinone (6PPD-Q) is a derivative of rubber tires which leaches into surface waters when tire particles are swept into roadway runoff. 6PPD-Q has been identified as a potential cause of urban runoff mortality syndrome in coho salmon, and subsequent research has determined a wide species variation in toxicity among fishes. While adult rainbow trout are known to be sensitive, there is limited research on their early-life stages. Given that early-life stages of fish are often more sensitive than adults, the aim of these studies was to assess the acute and sub-chronic toxicity of 6PPD-Q in early-life stage rainbow trout. Rainbow trout alevins were exposed from hatch until 28 days post-hatch (dph) to time-weighted average 6PPD-Q concentrations ranging from 0.06-2.35 μ g/L. From these studies, a 28-day median lethal dose (LC ₅₀ ) of 0.56 μ g/L was derived. Morphological deformities were observed during the exposure period, including pooling of blood in the caudal fin. A follow-up acute study with exogenously feeding rainbow trout fry revealed a 96-hour LC ₅₀ of 0.47 μ g/L. These studies indicate that early-life stage rainbow trout are more sensitive than previously studied sub-adults, with exogenously feeding fry being the most sensitive life stage studied so far, and that sub-chronic exposure to 6PPD-Q can result in developmental abnormalities. This research highlights the importance of utilizing early-life stage studies to determine the most sensitive benchmark concentrations and their value in determining sub-lethal effects.