Re-examination of the taxonomic status of the Antarctic Pseudomonas syringae Lz4W isolate and proposal to rename it as a novel species Pseudomonas cryophila sp. nov

A taxonomic re-evaluation of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W was performed in the light of its available genome sequence and due to a revision in the key phenotypic characteristics that are in conflict with the “ syringae ” group of Pseudomonads. A 16S rRNA gene sequence based phylogenetic analysis suggested that Lz4W ^T strain belongs to “ fragi ” cluster of Pseudomonas species, with closest similarity (99.72%) to the type strain P. deceptionensis M1 ^T . However, in silico analysis of the Lz4W genome sequence using SpecI (species identification tools), ANI (average nucleotide identity), and GBDP (Genome Blast Distance phylogeny) methods suggest that Lz4W ^T strain cannot be delineated with any of the type strains of “ fragi ” cluster of species. Based on predictive low DNA-DNA hybridization value (<29.9%) and differences in phenotypic features with the related species we suggest that Lz4W ^T is a novel species under the Pseudomonas genus, and we propose that the strain be named as Pseudomonas cryophila sp. nov. The type strain is Lz4W ^T (=CFBP 8403 ^T =KCTC 42933 ^T =LMG 29591 ^T =MTCC 673 ^T ).