In vitro Characterization of SARS-CoV-2 Protein Translated from the Moderna mRNA-1273 Vaccine

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   ScreenIT

   Mar 5, 2022

   SciScore for 10.1101/2022.03.01.22271618: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: All samples were acquired with approval from the Cedarville University Institutional Review Board. Field Sample Permit: Samples were collected in a collection vial containing 0.05% thimerosal.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Antibodies were incubated in concentration ranges from 1-10 μg/ml and detected using donkey anti-human secondary antibodies, (Jackson ImmunoResearch Laboratories; West Grove, PA) labeled with horse radish peroxidase (HRP) at a 1:5,000 dilution.	anti-human secondary antibodies, suggested: None
   The binding …

   SciScore for 10.1101/2022.03.01.22271618: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: All samples were acquired with approval from the Cedarville University Institutional Review Board. Field Sample Permit: Samples were collected in a collection vial containing 0.05% thimerosal.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Antibodies were incubated in concentration ranges from 1-10 μg/ml and detected using donkey anti-human secondary antibodies, (Jackson ImmunoResearch Laboratories; West Grove, PA) labeled with horse radish peroxidase (HRP) at a 1:5,000 dilution.	anti-human secondary antibodies, suggested: None
   The binding of the collected human saliva and REGEN-COV2 antibodies to cell lysates and supernatants was evaluated using an in-house developed ELISA.	REGEN-COV2 suggested: None
   To verify the actual molecular weights of the proteins, a mouse monoclonal antibody from R&D Systems, specific to the S2 subunit protein, (met697-Pro1213; cat# 1034617), was used at a concentration of 1 μg/ml diluted in Blotto (Santa Cruz Biotechnology, Dallas, TX).	S2 subunit protein, suggested: None
   In addition, western blotting with clarified saliva antibodies, was performed with samples diluted 1:3 in blotting-grade blocker, while the REGEN-COV humanized IgG1 antibodies were dissolved at 10 μg/ml in blotting-grade blocker.	humanized IgG1 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   2.1 Cell culture system to express SARS-CoV-2 protein(s) from synthetic mRNA-1273 vaccine (Spikevax): Vaccine uptake was tested in the normal mouse embryonic fibroblast NIH 3T3 adherent cell line (ATCC, Manassas, VA) and human monocytic U937 cells grown in suspension.	NIH 3T3 suggested: None U937 suggested: CLS Cat# 300368/p474_U-937, RRID:CVCL_0007)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Version published to 10.1101/2022.03.01.22271618v1 on medRxiv

   Mar 2, 2022