1. SciScore for 10.1101/2021.08.06.455405: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variableMice: B6.Cg-Tg(K18-ACE2)2Prlmn/J (K18-hACE2) were purchased from Jackson Laboratories and bred in-house at the University Hospital of Bonn. 8-to-20-week-old mice of both sexes were used throughout the study.
    Randomizationnot detected.
    BlindingThree scientists scored the sections in a blinded fashion as follows: 0 no staining; 1 weak staining, <5% brain area or <10% of lung area; 2 strong staining, 5-33% brain area or 10-50% of lung area; 3 strong staining >33% of brain area or >50% lung area.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    IgG ELISA: The presence of SARS-CoV-2-specific IgG antibodies in the sera of mice was quantified using ELISA (QuantiVac, Euroimmun) according to the manufacturer’s recommendations, with the exception that the anti-human anti-IgG antibody in the kit was exchanged for a goat anti-mouse IgG-HRP conjugate (Jackson Immuno Research, 115-035-062), 1:5000 in 0.5% BSA in PBS.
    SARS-CoV-2-specific IgG
    suggested: None
    anti-human anti-IgG
    suggested: None
    anti-mouse IgG-HRP
    suggested: None
    For SARS-CoV-2 antigen immunohistochemistry, slides were incubated with blocking reagent (10% normal goat serum) followed by rabbit monoclonal antibody against SARS-CoV-2 nucleocapsid protein (Biozol, SIN-40143-R019, 1:20000).
    SARS-CoV-2 nucleocapsid protein ( Biozol , SIN-40143-R019
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    The virus was passaged in VeroE6 cells and the viral titers were determined using a plaque assay as described in37.
    VeroE6
    suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
    Experimental Models: Organisms/Strains
    SentencesResources
    Mice: B6.Cg-Tg(K18-ACE2)2Prlmn/J (K18-hACE2) were purchased from Jackson Laboratories and bred in-house at the University Hospital of Bonn. 8-to-20-week-old mice of both sexes were used throughout the study.
    B6.Cg-Tg(K18-ACE2)2Prlmn/J
    suggested: RRID:IMSR_JAX:034860)
    K18-hACE2 transgenic mice were lightly anesthetized with ketamine/xylazine, before 5×104 PFU of SARS-CoV-2 virus was pipetted onto the nose and subsequently inhaled by the animal.
    K18-hACE2
    suggested: RRID:IMSR_GPT:T037657)
    Software and Algorithms
    SentencesResources
    Tissue sections were visualized using an Aperio SlideScanner CS2 and the Aperio Imagescope 12.4 software (Leica).
    Imagescope
    suggested: (ImageScope, RRID:SCR_014311)
    Statistical analysis: All statistical tests were calculated using Prism 9 (GraphPad).
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).


    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: We found the following clinical trial numbers in your paper:

    IdentifierStatusTitle
    NCT03739138CompletedIntratumoral/Intralesional Administration of MK-4621/JetPEI™…
    NCT0306502Trial number did not resolve on clinicaltrials.gov. Is the number correct?NA


    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.


    Results from JetFighter: We did not find any issues relating to colormaps.


    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.


    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

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