1. SciScore for 10.1101/2021.06.08.447224: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    NIH rigor criteria are not applicable to paper type.

    Table 2: Resources

    Software and Algorithms
    To overcome the input file limit of 50,000 amino acids per submission and handle sequences with non-standard amino acids, a Python script was developed.
    suggested: (IPython, RRID:SCR_001658)
    Statistical analysis and the generation of graphs was performed using GraphPad Prism (version 9.1.0) Structural Analysis: PDB metadata associated with proteins in the “Proteins With PDB” dataset that also contained a predicted Nsp5 cleavage (NetCorona score >0.5), were downloaded from the RCSB PDB website by generating a custom report in .csv format.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    Nsp5 cleavage sites predicted by NetCorona were matched with one PDB file per cleavage site, by searching the PDB metadata for the predicted 9 amino acid cleavage motif using Microsoft Excel (Additional File 6).
    Microsoft Excel
    suggested: (Microsoft Excel, RRID:SCR_016137)
    Publication quality figures were generated using PyMOL 2.3.0
    suggested: (PyMOL, RRID:SCR_000305)
    The node table (including tissue expression scores and compartments score for each protein) was exported to R for wrangling and data visualization using the tidyverse and ggrepel packages [94–96].
    suggested: (ggrepel, RRID:SCR_017393)
    Protein Network Analysis: The 48 proteins with a Nsp5 access score >500 and that had the potential to be found in the same cellular compartment as Nsp5 were imported into the STRING app (again within Cytoscape) while allowing a maximum of 5 additional interactor for the network generation instead of none.
    suggested: (STRING, RRID:SCR_005223)
    suggested: (Cytoscape, RRID:SCR_003032)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    N-terminomics based approaches have identified many potential Nsp5 cleavage sites in human proteins [47, 48], but they have some limitations that bioinformatics can compliment. Trypsin is used in the preparation of samples for mass spectrometry, which generates cleavages at lysine and arginine residues that are not N-terminal to a proline. Lysine and arginine appear in many cleavage sites predicted by NetCorona, meaning that cleavage by trypsin may mask true cleavage sites by artificially generating a N-terminus proximal to a P1 glutamine residue. Only one protein overlaps between the Koudelka et al. and Meyer et al. results, as these studies used different cell lines, and thus different proteins will be expressed, and the methods of exposure to Nsp5 also differed (cell lysate incubated with Nsp5 vs SARS-CoV-2 infection of cells) [47, 48]. Meyer et al. point out that the lysate-based method used by Koudelka et al. strips proteins of their subcellular context, which may lead to observed cleavage events that are not possible in vivo during infection [48]. In contrast, our bioinformatics analysis is cell-type and methodology agnostic as it examined the entire human proteome. The cleavage sites predicted in silico, combined with knowledge of Nsp5 subcellular localization and protein networks, identified several interesting human proteins and pathways. DHX15 contained a predicted cleavage site with the highest Nsp5 access score, and the protein may co-localize with Nsp5 in the nuc...

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

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