Circularly permuted LOV domain as an engineering module for optogenetic tools

  1. Lequn Geng
  2. Jiaqi Shen
  3. Wenjing Wang

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Abstract

We re-engineered a commonly-used light-sensing protein, LOV domain, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide. We demonstrate that the use of circularly permuted LOV domain on its own or together with the original LOV could expand the engineering capabilities of optogenetic tools.

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    Excerpt

    The circle of LOV: Circularly permuted LOV domain enables photo-control of proteins that require a free N-terminus and expands the applicability of optogenetic tools

  2. Version published to 10.1101/2021.05.19.444876v1 on bioRxiv
  3. Version published to 10.1039/d1cc02643g