Prevention and therapy of SARS-CoV-2 and the B.1.351 variant in mice

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   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2021.01.27.428478: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement

   IACUC: Biocontainment and biosafety: Studies were approved by the UNC Institutional Biosafety Committee approved by animal and experimental protocols in the Baric laboratory.

   Randomization

   In a blinded manner, three random fields of lung tissue were chosen and scored for the following: (A) neutrophils in the alveolar space (none = 0, 1–5 cells = 1, > 5 cells = 2), (B) neutrophils in the interstitial septae (none = 0, 1–5 cells = 1, > 5 cells = 2), (C) hyaline membranes (none = 0, one membrane = 1, > 1 membrane = 2), (D) Proteinaceous debris in air spaces (none = 0, one instance = 1, > 1 instance = 2), (E) alveolar septal thickening (< 2x mock thickness = 0, 2–4x …

   SciScore for 10.1101/2021.01.27.428478: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Biocontainment and biosafety: Studies were approved by the UNC Institutional Biosafety Committee approved by animal and experimental protocols in the Baric laboratory.
   Randomization	In a blinded manner, three random fields of lung tissue were chosen and scored for the following: (A) neutrophils in the alveolar space (none = 0, 1–5 cells = 1, > 5 cells = 2), (B) neutrophils in the interstitial septae (none = 0, 1–5 cells = 1, > 5 cells = 2), (C) hyaline membranes (none = 0, one membrane = 1, > 1 membrane = 2), (D) Proteinaceous debris in air spaces (none = 0, one instance = 1, > 1 instance = 2), (E) alveolar septal thickening (< 2x mock thickness = 0, 2–4x mock thickness = 1, > 4x mock thickness = 2).
   Blinding	Analyses and scoring were performed by a Board Certified Veterinary Pathologist who was blinded to the treatment groups as described previously (Sheahan et al., 2020a).
   Power Analysis	not detected.
   Sex as a biological variable	Study design and treatment groups: For the RDV experiment, a total of N=40, ~20-week-old male and female mice were divided into four groups each with N=10 mice with equal numbers of females and males in each group.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Groups of N=20 female mice (N=5 mice treated with C144 + C135, N=5 mice treated with C144, N=5 mice treated with C135, and N=5 mice treated with 3BNC117 + 10-1074) were administered antibody 12 hours before infection, N=20 female mice (N=5 mice treated with C144 + C135, N=5 mice treated with C144, N=5 mice treated with C135, and N=5 mice treated with 3BNC117 + 10-1074) were administered antibody 12hpi (early therapeutic group), N=20 female mice (N=5 mice treated with C144 + C135, N=5 mice treated with C144, N=5 mice treated with C135, and N=5 mice treated with 3BNC117 + 10-1074) were administered antibody 24hpi (mid-late therapeutic group), and N=20 female mice (N=5 mice treated with C144 + C135, N=5 mice treated with C144, N=5 mice treated with C135, and N=5 mice treated with 3BNC117 + 10-1074) were administered antibody 48hpi (late therapeutic group).	C135 suggested: None
   Software and Algorithms
   Sentences	Resources
   Statistics: All statistical analyses were performed using GraphPad Prism 9.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2021.01.27.428478 on bioRxiv

   Jan 28, 2021