Efficacy of GC-376 against SARS-CoV-2 virus infection in the K18 hACE2 transgenic mouse model

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   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2021.01.27.428428: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Ethics Statement: Studies were approved by the Institutional Animal Care and Use Committee.
   Randomization	Mice were randomly distributed in the number of groups (Fig 1A), anesthetized with isoflurane and challenged intranasally (i.n.) with 50 µl of either phosphate buffer saline (PBS), or 1×103 TCID50/mouse or 1×105 TCID50/mouse.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Mouse studies: 6-week-old female K18-hACE2 mice were purchased from Jackson laboratories (Bar Harbor, ME).
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Viral stocks were prepared in Vero E6 Pasteur …

   SciScore for 10.1101/2021.01.27.428428: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Ethics Statement: Studies were approved by the Institutional Animal Care and Use Committee.
   Randomization	Mice were randomly distributed in the number of groups (Fig 1A), anesthetized with isoflurane and challenged intranasally (i.n.) with 50 µl of either phosphate buffer saline (PBS), or 1×103 TCID50/mouse or 1×105 TCID50/mouse.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Mouse studies: 6-week-old female K18-hACE2 mice were purchased from Jackson laboratories (Bar Harbor, ME).
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Viral stocks were prepared in Vero E6 Pasteur cells.	Vero E6 suggested: RRID:CVCL_XD71)
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Mouse studies: 6-week-old female K18-hACE2 mice were purchased from Jackson laboratories (Bar Harbor, ME).	K18-hACE2 suggested: RRID:IMSR_GPT:T037657)
   Software and Algorithms
   Sentences	Resources
   Graphs/Statistical analyses: All data analyses and graphs were performed using GraphPad Prism software version 8 (	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)
   GraphPad Software Inc.	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
   

   Identifier	Status	Title
   NCT04426695	Recruiting	Safety, Tolerability, and Efficacy of Anti-Spike (S) SARS-Co…

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2021.01.27.428428 on bioRxiv

   Jan 27, 2021