Whole Genome Sequencing and in vitro analyses highlight the production of folate and the antimicrobial properties of Lacticaseibacillus rhamnosus SL42.

Lacticaseibacillus rhamnosus SL42 is a probiotic strain isolated Whole Genome Sequencing (WGS) and in vitro analyses were used to assess the probiotic potential of Lacticaseibacillus rhamnosus SL42, a breast milk isolate, for B-vitamin production and antimicrobial property. Genomic analysis found genes involved in vitamin B9 biosynthesis and defense mechanisms, indicating the strain's potential for use in food biotechnology. This was supported by in vitro confirmations. SL42 was able to synthesize folate in milk, demonstrating the functioning of the coding genes. PathogenFinder discovered that the strain lacks the pathogenic-associated genes; suggesting its suitability as a probiotic and biofortifying agent in food products. Furthermore, a plethora of coding genes annotated for defense and adaptability to numerous harsh conditions, as well as functional properties associated with antioxidant activity and bioremediation, were discovered. The strain reflects an exceptional adaptability in a multiplicity of situations, most specifically cold-chain storages. In general, SL42's beneficial role has been linked to different biotechnological and therapeutic applications mainly B-vitamin biofortification, immune diseases, and neurodegenerative disorders.