Optimization of surface sterilization method to isolate endophytic bacteria and fungi from the fine root of Encelia farinosa
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Desert plants recruit beneficial endophytic microbes and play an important role in ecosystem conversation. To recover these microbes, epiphytic microbes must be eliminated through the efficiency of surface sterilization. In the current study, different concentrations of sterilant with varying exposure times have been assessed to isolate fungal and bacterial endophytes from the fine root of Encelia farinosa. The methods involved washing fine roots with various concentrations of sodium hypochlorite for different exposure times to optimize surface sterilization for isolating endophytes. The result indicates that treatment 1 of 0.1% sodium hypochlorite for 1 minute was found effective. Future research will identify bacteria and fungi that can promote growth, provide biological control, and produce enzymes.
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Thank you for your submission. This work presents a useful finding that would be a valuable contribution to the field. However, before I send this article out for peer review, a few adjustments are needed. Firstly, it is important to include more details on the methodology. How many replicates did you perform for each treatment condition? After you perform the wash with sodium hypochlorite, you write that you wash with sterilised distilled water up to 3 times. It would be good to standardise this into a number of washes. In your methods, you state that the isolation of bacteria was only carried out on roots treated with treatment 1. Therefore, in Table 1, you cannot conclude that there was no growth of microbes following treatment 2-4 as you have not tested for bacterial growth. It would therefore be important to also test the …
Thank you for your submission. This work presents a useful finding that would be a valuable contribution to the field. However, before I send this article out for peer review, a few adjustments are needed. Firstly, it is important to include more details on the methodology. How many replicates did you perform for each treatment condition? After you perform the wash with sodium hypochlorite, you write that you wash with sterilised distilled water up to 3 times. It would be good to standardise this into a number of washes. In your methods, you state that the isolation of bacteria was only carried out on roots treated with treatment 1. Therefore, in Table 1, you cannot conclude that there was no growth of microbes following treatment 2-4 as you have not tested for bacterial growth. It would therefore be important to also test the bacteria isolation following treatments 2-4. Moreover, some quantification of your results would be important to test your hypothesis and make this work more robust. Perhaps you could count the number of bacterial colonies that form following each treatment type. Finally, to widen the audience of this work, it would be good for you to identify the type of endophytic fungi or bacteria that you can cultivate from these plants through morphological or molecular methods.
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